• STJ73246 (0. 03µg/ml) staining of Human Heart (A) , Kidney (B) and Liver (C) lysate and in Pig Heart (D) , Kidney (E) and Liver (F) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ73246 (0. 1µg/ml) staining of Rat Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ73246 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Anti-HSP60/HSPD1 antibody (333-344) (STJ73246)

SKU:
STJ73246-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF
Reactivity: Human/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-HSP60/HSPD1 (333-344) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-5µg/ml
IF-Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: HSPD1
Gene ID: 3329
Uniprot ID: CH60_HUMAN
Immunogen Region: 333-344
Accession Number: NP_002147.2
Specificity: Reported variants represent identical protein: NP_955472.1, NP_002147.2.
Immunogen Sequence: NLEDVQPHDLGK
Protein Name 60 Kda Heat Shock Protein - Mitochondrial
60 Kda Chaperonin
Chaperonin 60
Cpn60
Heat Shock Protein 60
Hsp-60
Hsp60
Hucha60
Mitochondrial Matrix Protein P1
P60 Lymphocyte Protein
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-8869496
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-60 Kda Heat Shock Protein - Mitochondrial antibody
Anti-60 Kda Chaperonin antibody
Anti-Chaperonin 60 antibody
Anti-Cpn60 antibody
Anti-Heat Shock Protein 60 antibody
Anti-Hsp-60 antibody
Anti-Hsp60 antibody
Anti-Hucha60 antibody
Anti-Mitochondrial Matrix Protein P1 antibody
Anti-P60 Lymphocyte Protein antibody
Anti-HSPD1 antibody
Anti-HSP60 antibody

Information sourced from Uniprot.org

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