| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Hypoxanthine-Guanine Phosphoribosyltransferase (1-218) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HPRT1 |
| Gene ID: |
3251 |
| Uniprot ID: |
HPRT_HUMAN |
| Immunogen Region: |
1-218 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human HPRT1 (NP_000185.1). |
| Immunogen Sequence: |
MATRSPGVVISDDEPGYDLD LFCIPNHYAEDLERVFIPHG LIMDRTERLARDVMKEMGGH HIVALCVLKGGYKFFADLLD YIKALNRNSDRSIPMTVDFI RLKSYCNDQSTGDIKVIGGD DLSTLTGKNVLIVEDIIDTG KTMQTLLSLVRQYNPKMVKV ASLLVKRTPRSVGYKPDFVG FEIPDKFVVGYALDYNEYFR DLNHVCVISETGKAKYKA |
| Function | Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. |
| Protein Name | Hypoxanthine-Guanine PhosphoribosyltransferaseHgprtHgprtase |
| Database Links | Reactome: R-HSA-74217Reactome: R-HSA-9734281Reactome: R-HSA-9748787 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Hypoxanthine-Guanine Phosphoribosyltransferase antibodyAnti-Hgprt antibodyAnti-Hgprtase antibodyAnti-HPRT1 antibodyAnti-HPRT antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and HPRT1 knockout (KO) 293T cells, using [KO Validated] HPRT1 Rabbit polyclonal antibody (STJ27659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/101287/388854/STJ27659_1__09600.1713158416.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HPRT1 knockout (KO) 293T cells, using [KO Validated] HPRT1 Rabbit polyclonal antibody (STJ27659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
