![Western blot analysis of lysates from wild type (WT) and HPRT1 knockout (KO) 293T cells, using [KO Validated] HPRT1 Rabbit mAb (STJ11102375) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91292/406016/STJ11102375_2__21777.1744891806.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HPRT1 knockout (KO) 293T cells, using [KO Validated] HPRT1 Rabbit mAb (STJ11102375) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.")
Anti-HPRT1 antibody (119-218) [S5MR] (STJ11102375)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal HPRT (119-218) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HPRT1 |
| Gene ID | 3251 |
| Uniprot ID | HPRT_HUMAN |
| Immunogen Region | 119-218 |
| Immunogen Sequence | GDDLSTLTGKNVLIVEDIID TGKTMQTLLSLVRQYNPKMV KVASLLVKRTPRSVGYKPDF VGFEIPDKFVVGYALDYNEY FRDLNHVCVISETGKAKYKA |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 119-218 of human HPRT11 (NP_000185.1). |
Additional Info
| Function | Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. |
| Protein Name | Hypoxanthine-Guanine PhosphoribosyltransferaseHgprtHgprtase |
| Database Links | Reactome: R-HSA-74217Reactome: R-HSA-9734281Reactome: R-HSA-9748787 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Hypoxanthine-Guanine Phosphoribosyltransferase antibodyAnti-Hgprt antibodyAnti-Hgprtase antibodyAnti-HPRT1 antibodyAnti-HPRT antibody |
Information sourced from Uniprot.org