Anti-HOMER1 antibody (125-354 aa) (STJ11107406)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal HOMER1 (125-354 aa) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HOMER1 |
| Gene ID | 9456 |
| Uniprot ID | HOME1_HUMAN |
| Immunogen Region | 125-354 aa |
| Immunogen Sequence | STPSQESAGGDLQSPLTPES INGTDDERTPDVTQNSEPRA EPTQNALPFSHSSAISKHWE AELATLKGNNAKLTAALLES TANVKQWKQQLAAYQEEAER LHKRVTELECVSSQANAVHT HKTELNQTIQELEETLKLKE EEIERLKQEIDNARELQEQR DSLTQKLQEVEIRNKDLEGQ LSDLEQRLEKSQNEQEAFRN NLKTLLEILDGKIFELTELR DNLAKLLECS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 125-354 of human HOMER1 (NP_004263.1). |
Additional Info
| Function | Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines. Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA. |
| Protein Name | Homer Protein Homolog 1Homer-1 |
| Database Links | Reactome: R-HSA-6794361 |
| Cellular Localisation | CytoplasmPostsynaptic DensitySynapseCell ProjectionDendritic SpineIsoform 1 Inhibits Surface Expression Of Grm5 Causing It To Be Retained In The Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Homer Protein Homolog 1 antibodyAnti-Homer-1 antibodyAnti-HOMER1 antibodyAnti-SYN47 antibody |
Information sourced from Uniprot.org