• Western blot analysis of extracts of various cell lines, using HNRNPF antibody (STJ27456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts from wild type (WT) and HNRNPF knockout (KO) 293T cells, using HNRNPF antibody (STJ27456) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of U2OS cells using HNRNPF antibody (STJ27456). Blue: DAPI for nuclear staining.

Anti-HNRNPF antibody (1-280) (STJ27456)

SKU:
STJ27456

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HNRNPF (1-280) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNRNPF
Gene ID: 3185
Uniprot ID: HNRPF_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HNRNPF (NP_001091678.1).
Immunogen Sequence: MMLGPEGGEGFVVKLRGLPW SCSVEDVQNFLSDCTIHDGA AGVHFIYTREGRQSGEAFVE LGSEDDVKMALKKDRESMGH RYIEVFKSHRTEMDWVLKHS GPNSADSANDGFVRLRGLPF GCTKEEIVQFFSGLEIVPNG ITLPVDPEGKITGEAFVQFA SQELAEKALGKHKERIGHRY IEVFKSSQEEVRSYSDPPLK FMSVQRPGPYDRPGTARRYI GIVKQAGLERMRPGAYSTG
Tissue Specificity Expressed ubiquitously.
Post Translational Modifications Sumoylated.
Function Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.
Protein Name Heterogeneous Nuclear Ribonucleoprotein F
Hnrnp F
Nucleolin-Like Protein Mcs94-1 Cleaved Into - Heterogeneous Nuclear Ribonucleoprotein F - N-Terminally Processed
Database Links Reactome: R-HSA-6803529
Reactome: R-HSA-72163
Reactome: R-HSA-72203
Reactome: R-HSA-8950505
Cellular Localisation Nucleus
Nucleoplasm
Alternative Antibody Names Anti-Heterogeneous Nuclear Ribonucleoprotein F antibody
Anti-Hnrnp F antibody
Anti-Nucleolin-Like Protein Mcs94-1 Cleaved Into - Heterogeneous Nuclear Ribonucleoprotein F - N-Terminally Processed antibody
Anti-HNRNPF antibody
Anti-HNRPF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance