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Anti-HNRNPF antibody (1-280) (STJ27456)

Western blot analysis of extracts of various cell lines, using HNRNPF antibody (STJ27456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western blot analysis of extracts from wild type (WT) and HNRNPF knockout (KO) 293T cells, using HNRNPF antibody (STJ27456) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunofluorescence analysis of U2OS cells using HNRNPF antibody (STJ27456). Blue: DAPI for nuclear staining.

Anti-HNRNPF antibody (1-280) (STJ27456)

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STJ27456

£129.50 - £639.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IF
Reactivity:	Human/Mouse
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-HNRNPF (1-280) is suitable for use in Western Blot and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:1000-1:5000 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	HNRNPF
Gene ID:	3185
Uniprot ID:	HNRPF_HUMAN
Immunogen Region:	1-280
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HNRNPF (NP_001091678.1).
Immunogen Sequence:	MMLGPEGGEGFVVKLRGLPW SCSVEDVQNFLSDCTIHDGA AGVHFIYTREGRQSGEAFVE LGSEDDVKMALKKDRESMGH RYIEVFKSHRTEMDWVLKHS GPNSADSANDGFVRLRGLPF GCTKEEIVQFFSGLEIVPNG ITLPVDPEGKITGEAFVQFA SQELAEKALGKHKERIGHRY IEVFKSSQEEVRSYSDPPLK FMSVQRPGPYDRPGTARRYI GIVKQAGLERMRPGAYSTG

Tissue Specificity	Expressed ubiquitously.
Post Translational Modifications	Sumoylated.
Function	Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.
Protein Name	Heterogeneous Nuclear Ribonucleoprotein F Hnrnp F Nucleolin-Like Protein Mcs94-1 Cleaved Into - Heterogeneous Nuclear Ribonucleoprotein F - N-Terminally Processed
Database Links	Reactome: R-HSA-6803529 Reactome: R-HSA-72163 Reactome: R-HSA-72203 Reactome: R-HSA-8950505
Cellular Localisation	Nucleus Nucleoplasm
Alternative Antibody Names	Anti-Heterogeneous Nuclear Ribonucleoprotein F antibody Anti-Hnrnp F antibody Anti-Nucleolin-Like Protein Mcs94-1 Cleaved Into - Heterogeneous Nuclear Ribonucleoprotein F - N-Terminally Processed antibody Anti-HNRNPF antibody Anti-HNRPF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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