• Western blot analysis of various lysates, using HMGCS2 rabbit polyclonal antibody (STJ116457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using HMGCS2 antibody (STJ116457) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using HMGCS2 antibody (STJ116457) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat liver using HMGCS2 Polyclonal Antibody (STJ116457) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human liver using HMGCS2 Polyclonal Antibody (STJ116457) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse liver using HMGCS2 Polyclonal Antibody (STJ116457) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Anti-HMGCS2 antibody (269-508) (STJ116457)

Anti-HMGCS2 antibody (269-508) (STJ116457)

SKU:
STJ116457

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HMGCS2 (269-508) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMGCS2
Gene ID: 3158
Uniprot ID: HMCS2_HUMAN
Immunogen Region: 269-508
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 269-508 of human HMGCS2 (NP_005509.1).
Immunogen Sequence: CYTSYRKKIQNQWKQAGSDR PFTLDDLQYMIFHTPFCKMV QKSLARLMFNDFLSASSDTQ TSLYKGLEAFGGLKLEDTYT NKDLDKALLKASQDMFDKKT KASLYLSTHNGNMYTSSLYG CLASLLSHHSAQELAGSRIG AFSYGSGLAASFFSFRVSQD AAPGSPLDKLVSSTSDLPKR LASRKCVSPEEFTEIMNQRE QFYHKVNFSPPGDTNSLFPG TWYLERVDEQHRRKYARRP
Tissue Specificity Expression in liver is 200-fold higher than in any other tissue. Low expression in colon, kidney, testis, and pancreas. Very low expression in heart and skeletal muscle. Not detected in brain. Isoform 3: Highest expression detected in heart and skeletal muscle.
Post Translational Modifications Succinylated. Desuccinylated by SIRT5. Succinylation, at least at Lys-83 and Lys-310, inhibits the enzymatic activity.
Function Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate.
Protein Name Hydroxymethylglutaryl-Coa Synthase - Mitochondrial
Hmg-Coa Synthase
3-Hydroxy-3-Methylglutaryl Coenzyme A Synthase
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-77111
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Hydroxymethylglutaryl-Coa Synthase - Mitochondrial antibody
Anti-Hmg-Coa Synthase antibody
Anti-3-Hydroxy-3-Methylglutaryl Coenzyme A Synthase antibody
Anti-HMGCS2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance