• Immunofluorescence analysis of HeLa cells using HMGCS1 Rabbit polyclonal antibody (STJ24047) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using HMGCS1 Rabbit polyclonal antibody (STJ24047) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using HMGCS1 Rabbit polyclonal antibody (STJ24047) at 1:7000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HMGCS1 antibody (241-520) (STJ24047)

SKU:
STJ24047

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HMGCS1 (241-520) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMGCS1
Gene ID: 3157
Uniprot ID: HMCS1_HUMAN
Immunogen Region: 241-520
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 241-520 of human HMGCS1 (NP_002121.4).
Immunogen Sequence: HAQWQKEGNDKDFTLNDFGF MIFHSPYCKLVQKSLARMLL NDFLNDQNRDKNSIYSGLEA FGDVKLEDTYFDRDVEKAFM KASSELFSQKTKASLLVSNQ NGNMYTSSVYGSLASVLAQY SPQQLAGKRIGVFSYGSGLA ATLYSLKVTQDATPGSALDK ITASLCDLKSRLDSRTGVAP DVFAENMKLREDTHHLVNYI PQGSIDSLFEGTWYLVRVDE KHRRTYARRPTPNDDTLDE
Function Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate, a precursor for cholesterol synthesis.
Protein Name Hydroxymethylglutaryl-Coa Synthase - Cytoplasmic
Hmg-Coa Synthase
3-Hydroxy-3-Methylglutaryl Coenzyme A Synthase
Database Links Reactome: R-HSA-191273
Reactome: R-HSA-1989781
Reactome: R-HSA-2426168
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Hydroxymethylglutaryl-Coa Synthase - Cytoplasmic antibody
Anti-Hmg-Coa Synthase antibody
Anti-3-Hydroxy-3-Methylglutaryl Coenzyme A Synthase antibody
Anti-HMGCS1 antibody
Anti-HMGCS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance