• STJ71766 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71766 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-HMGCR/LDLCQ3 antibody (Internal) (STJ71766)

SKU:
STJ71766-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-HMGCR/LDLCQ3 (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the endoplasmic reticulum and vesicles of A549 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: HMGCR
Gene ID: 3156
Uniprot ID: HMDH_HUMAN
Immunogen Region: Internal
Accession Number: NP_000850.1; NP_001124468.1
Immunogen Sequence: ADPSPQNSTADTSK
Post Translational Modifications N-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner. Undergoes sterol-mediated ubiquitination and ER-associated degradation (ERAD). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1 or INSIG2. The INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78, RNF139 or RNF145, initiating ubiquitination of the reductase. The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97. The INSIG2-binding leads to the recruitment of the ubiquitin ligase RNF139, initiating ubiquitination of the reductase. Lys-248 is the main site of ubiquitination. Ubiquitination is enhanced by the presence of a geranylgeranylated protein. Phosphorylated. Phosphorylation at Ser-872 reduces the catalytic activity.
Function Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis. HMGCR is the main target of statins, a class of cholesterol-lowering drugs.
Protein Name 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase
Hmg-Coa Reductase
Database Links Reactome: R-HSA-191273
Reactome: R-HSA-1989781 P04035-1
Reactome: R-HSA-2426168 P04035-1
Reactome: R-HSA-9619665
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Peroxisome Membrane
Alternative Antibody Names Anti-3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase antibody
Anti-Hmg-Coa Reductase antibody
Anti-HMGCR antibody

Information sourced from Uniprot.org

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