Anti-HMGCR/LDLCQ3 antibody (Internal) (STJ71766)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-HMGCR/LDLCQ3 (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications. |
| Applications | Pep-ELISA/IF/FC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.IF: Strong expression of the protein seen in the endoplasmic reticulum and vesicles of A549 cells. Recommended concentration: 10µg/ml. FC:Flow cytometric analysis of A549 cells. Recommend |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | HMGCR |
| Gene ID | 3156 |
| Uniprot ID | HMDH_HUMAN |
| Accession Number | NP_000850.1; NP_001124468.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | ADPSPQNSTADTSK |
Additional Info
| Post Translational Modifications | N-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner. Undergoes sterol-mediated ubiquitination and ER-associated degradation (ERAD). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1 or INSIG2. The INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78, RNF139 or RNF145, initiating ubiquitination of the reductase. The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97. The INSIG2-binding leads to the recruitment of the ubiquitin ligase RNF139, initiating ubiquitination of the reductase. Lys-248 is the main site of ubiquitination. Ubiquitination is enhanced by the presence of a geranylgeranylated protein. Phosphorylated. Phosphorylation at Ser-872 reduces the catalytic activity. |
| Function | Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis. HMGCR is the main target of statins, a class of cholesterol-lowering drugs. |
| Protein Name | 3-Hydroxy-3-Methylglutaryl-Coenzyme A ReductaseHmg-Coa Reductase |
| Database Links | Reactome: R-HSA-191273Reactome: R-HSA-1989781 P04035-1Reactome: R-HSA-2426168 P04035-1Reactome: R-HSA-9619665 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinPeroxisome Membrane |
| Alternative Antibody Names | Anti-3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase antibodyAnti-Hmg-Coa Reductase antibodyAnti-HMGCR antibody |
Information sourced from Uniprot.org