| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-High mobility group protein B3 (123-173 aa) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HMGB3 |
| Gene ID: |
3149 |
| Uniprot ID: |
HMGB3_HUMAN |
| Immunogen Region: |
123-173 aa |
| Specificity: |
This antibody detects endogenous levels of HMGB3 at Human/Mouse |
| Immunogen: |
Synthesized peptide derived from the human HMGB3 at the amino acid range 123-173 |
| Post Translational Modifications | Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-104 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB3 (HMGB3C23hC45hC104h), 2- disulfide HMGB3 (HMGB3C23-C45C104h) and 3- sulfonyl HMGB3 (HMGB3C23soC45soC104so). |
| Function | Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference for non-canonical DNA structures such as single-stranded DNA. Can bend DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters. Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor. Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling. |
| Protein Name | High Mobility Group Protein B3High Mobility Group Protein 2aHmg-2aHigh Mobility Group Protein 4Hmg-4 |
| Cellular Localisation | NucleusChromosomeCytoplasm |
| Alternative Antibody Names | Anti-High Mobility Group Protein B3 antibodyAnti-High Mobility Group Protein 2a antibodyAnti-Hmg-2a antibodyAnti-High Mobility Group Protein 4 antibodyAnti-Hmg-4 antibodyAnti-HMGB3 antibodyAnti-HMG2A antibodyAnti-HMG4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
