Host: | Rabbit |
Applications: | WB |
Reactivity: | Human/Mouse |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-High mobility group protein B3 (123-173 aa) is suitable for use in Western Blot research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration: | 1 mg/mL |
Dilution Range: | WB 1:500-2000 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | HMGB3 |
Gene ID: | 3149 |
Uniprot ID: | HMGB3_HUMAN |
Immunogen Region: | 123-173 aa |
Specificity: | This antibody detects endogenous levels of HMGB3 at Human/Mouse |
Immunogen: | Synthesized peptide derived from the human HMGB3 at the amino acid range 123-173 |
Post Translational Modifications | Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-104 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB3 (HMGB3C23hC45hC104h), 2- disulfide HMGB3 (HMGB3C23-C45C104h) and 3- sulfonyl HMGB3 (HMGB3C23soC45soC104so). |
Function | Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference for non-canonical DNA structures such as single-stranded DNA. Can bend DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters. Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor. Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling. |
Protein Name | High Mobility Group Protein B3 High Mobility Group Protein 2a Hmg-2a High Mobility Group Protein 4 Hmg-4 |
Cellular Localisation | Nucleus Chromosome Cytoplasm |
Alternative Antibody Names | Anti-High Mobility Group Protein B3 antibody Anti-High Mobility Group Protein 2a antibody Anti-Hmg-2a antibody Anti-High Mobility Group Protein 4 antibody Anti-Hmg-4 antibody Anti-HMGB3 antibody Anti-HMG2A antibody Anti-HMG4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance