| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Abasic site processing protein HMCES (173-223 aa) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HMCES |
| Gene ID: |
56941 |
| Uniprot ID: |
HMCES_HUMAN |
| Immunogen Region: |
173-223 aa |
| Specificity: |
This antibody detects endogenous levels of CC037 at Human/Mouse/Rat |
| Immunogen: |
Synthesized peptide derived from the human CC037 at the amino acid range 173-223 |
| Function | Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites. Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue. The HMCES DNA-protein cross-link is then degraded by the proteasome. Promotes error-free repair of abasic sites by acting as a 'suicide' enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks. Has preference for ssDNA, but can also accommodate double-stranded DNA with 3' or 5' overhang (dsDNA), and dsDNA-ssDNA 3' junction. Also involved in class switch recombination (CSR) in B-cells independently of the formation of a DNA-protein cross-link: acts by binding and protecting ssDNA overhangs to promote DNA double-strand break repair through the microhomology-mediated alternative-end-joining (Alt-EJ) pathway. Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine. |
| Protein Name | Abasic Site Processing Protein HmcesEmbryonic Stem Cell-Specific 5-Hydroxymethylcytosine-Binding ProteinEs Cell-Specific 5hmc-Binding ProteinPeptidase HmcesSrap Domain-Containing Protein 1 |
| Cellular Localisation | ChromosomeRecruited To Chromatin Following Dna DamageLocalizes To Replication Forks |
| Alternative Antibody Names | Anti-Abasic Site Processing Protein Hmces antibodyAnti-Embryonic Stem Cell-Specific 5-Hydroxymethylcytosine-Binding Protein antibodyAnti-Es Cell-Specific 5hmc-Binding Protein antibodyAnti-Peptidase Hmces antibodyAnti-Srap Domain-Containing Protein 1 antibodyAnti-HMCES antibodyAnti-C3orf37 antibodyAnti-DC12 antibodyAnti-SRAPD1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
