• Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HMCES antibody (173-223 aa) (STJ193508)

SKU:
STJ193508

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Abasic site processing protein HMCES (173-223 aa) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMCES
Gene ID: 56941
Uniprot ID: HMCES_HUMAN
Immunogen Region: 173-223 aa
Specificity: This antibody detects endogenous levels of CC037 at Human/Mouse/Rat
Immunogen: Synthesized peptide derived from the human CC037 at the amino acid range 173-223
Function Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites. Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue. The HMCES DNA-protein cross-link is then degraded by the proteasome. Promotes error-free repair of abasic sites by acting as a 'suicide' enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks. Has preference for ssDNA, but can also accommodate double-stranded DNA with 3' or 5' overhang (dsDNA), and dsDNA-ssDNA 3' junction. Also involved in class switch recombination (CSR) in B-cells independently of the formation of a DNA-protein cross-link: acts by binding and protecting ssDNA overhangs to promote DNA double-strand break repair through the microhomology-mediated alternative-end-joining (Alt-EJ) pathway. Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine.
Protein Name Abasic Site Processing Protein Hmces
Embryonic Stem Cell-Specific 5-Hydroxymethylcytosine-Binding Protein
Es Cell-Specific 5hmc-Binding Protein
Peptidase Hmces
Srap Domain-Containing Protein 1
Cellular Localisation Chromosome
Recruited To Chromatin Following Dna Damage
Localizes To Replication Forks
Alternative Antibody Names Anti-Abasic Site Processing Protein Hmces antibody
Anti-Embryonic Stem Cell-Specific 5-Hydroxymethylcytosine-Binding Protein antibody
Anti-Es Cell-Specific 5hmc-Binding Protein antibody
Anti-Peptidase Hmces antibody
Anti-Srap Domain-Containing Protein 1 antibody
Anti-HMCES antibody
Anti-C3orf37 antibody
Anti-DC12 antibody
Anti-SRAPD1 antibody

Information sourced from Uniprot.org

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