Anti-HMBS antibody (1-361) (STJ24034)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal HMBS (1-361) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HMBS |
| Gene ID | 3145 |
| Uniprot ID | HEM3_HUMAN |
| Immunogen Region | 1-361 |
| Immunogen Sequence | MSGNGNAAATAEENSPKMRV IRVGTRKSQLARIQTDSVVA TLKASYPGLQFEIIAMSTTG DKILDTALSKIGEKSLFTKE LEHALEKNEVDLVVHSLKDL PTVLPPGFTIGAICKRENPH DAVVFHPKFVGKTLETLPEK SVVGTSSLRRAAQLQRKFPH LEFRSIRGNLNTRLRKLDEQ QEFSAIILATAGLQRMGWHN RVGQILHPEECMYAVGQGAL GVEVRAKDQDILDLVGVLH |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-361 of human HMBS (NP_000181.2). |
Additional Info
| Tissue Specificity | Isoform 1: Is ubiquitously expressed. Isoform 2: Is found only in erythroid cells. |
| Function | As part of the heme biosynthetic pathway, catalyzes the sequential polymerization of four molecules of porphobilinogen to form hydroxymethylbilane, also known as preuroporphyrinogen. Catalysis begins with the assembly of the dipyrromethane cofactor by the apoenzyme from two molecules of porphobilinogen or from preuroporphyrinogen. The covalently linked cofactor acts as a primer, around which the tetrapyrrole product is assembled. In the last step of catalysis, the product, preuroporphyrinogen, is released, leaving the cofactor bound to the holodeaminase intact. |
| Protein Name | Porphobilinogen DeaminasePbg-DHydroxymethylbilane SynthaseHmbsPre-Uroporphyrinogen Synthase |
| Database Links | Reactome: R-HSA-189451 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Porphobilinogen Deaminase antibodyAnti-Pbg-D antibodyAnti-Hydroxymethylbilane Synthase antibodyAnti-Hmbs antibodyAnti-Pre-Uroporphyrinogen Synthase antibodyAnti-HMBS antibodyAnti-PBGD antibodyAnti-UPS antibody |
Information sourced from Uniprot.org