| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Histone H1t (110-209) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
H1-6 |
| Gene ID: |
107970 |
| Uniprot ID: |
H1T_MOUSE |
| Immunogen Region: |
110-209 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 110-209 of mouse Histone H1t (NP_034507.2). |
| Immunogen Sequence: |
LSKKAASGNDKGKGKKSASA KAKKMGLPRASRSPKSSKTK AVKKPKATPTKASGSGRKTK GAKGVQQRKSPAKARAANPN SGKAKMVMQKTDLRKAAGRK |
| Tissue Specificity | Testis-specific. |
| Post Translational Modifications | Phosphorylated in early spermatids. Citrullination at Arg-56 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. |
| Function | Testis-specific histone H1 that forms less compacted chromatin compared to other H1 histone subtypes. Formation of more relaxed chromatin may be required to promote chromatin architecture required for proper chromosome regulation during meiosis, such as homologous recombination. Histones H1 act as linkers that bind to nucleosomes and compact polynucleosomes into a higher-order chromatin configuration. |
| Protein Name | Histone H1tTesticular H1 Histone |
| Cellular Localisation | NucleusChromosome |
| Alternative Antibody Names | Anti-Histone H1t antibodyAnti-Testicular H1 Histone antibodyAnti-H1-6 antibodyAnti-H1f6 antibodyAnti-H1ft antibodyAnti-H1t antibodyAnti-Hist1h1t antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
