• Western blot analysis of extracts of K-562 cells, using HFM1 antibody (STJ111332) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using HFM1 antibody (STJ111332) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat testis using HFM1 antibody (STJ111332) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat testis using HFM1 antibody (STJ111332) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse testis using HFM1 antibody (STJ111332) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of K-562 cells, using HFM1 antibody (STJ111332) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HFM1 antibody (1-240) (STJ111332)

SKU:
STJ111332

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HFM1 (1-240) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HFM1
Gene ID: 164045
Uniprot ID: HFM1_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HFM1 (NP_001017975.4).
Immunogen Sequence: MLKSNDCLFSLENLFFEKPD EVENHPDNEKSLDWFLPPAP LISEIPDTQELEEELESHKL LGQEKRPKMLTSNLKITNED TNYISLTQKFQFAFPSDKYE QDDLNLEGVGNNDLSHVAGK LTYASQKYKNHIGTEIAPEK SVPDDTKLVNFAEDKGESTS VFRKRLFKISDNIHGSAYSN DNELDSHIGSVKIVQTEMNK GKSRNYSNSKQKFQYSANVF TANNAFSASEIGEGMFKAP
Tissue Specificity Preferentially expressed in testis and ovary.
Function Required for crossover formation and complete synapsis of homologous chromosomes during meiosis.
Protein Name Probable Atp-Dependent Dna Helicase Hfm1
Sec63 Domain-Containing Protein 1
Alternative Antibody Names Anti-Probable Atp-Dependent Dna Helicase Hfm1 antibody
Anti-Sec63 Domain-Containing Protein 1 antibody
Anti-HFM1 antibody
Anti-SEC3D1 antibody

Information sourced from Uniprot.org

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