• Western blot analysis of various lysates using HEPACAM Rabbit polyclonal antibody (STJ113194) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HEPACAM antibody (35-245) (STJ113194)

SKU:
STJ113194

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HEPACAM (35-245) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HEPACAM
Gene ID: 220296
Uniprot ID: HECAM_HUMAN
Immunogen Region: 35-245
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-245 of human HEPACAM (NP_689935.2).
Immunogen Sequence: NITSPVRLIHGTVGKSALLS VQYSSTSSDRPVVKWQLKRD KPVTVVQSIGTEVIGTLRPD YRDRIRLFENGSLLLSDLQL ADEGTYEVEISITDDTFTGE KTINLTVDVPISRPQVLVAS TTVLELSEAFTLNCSHENGT KPSYTWLKDGKPLLNDSRML LSPDQKVLTITRVLMEDDDL YSCMVENPISQGRSLPVKIT VYRRSSLYIIL
Post Translational Modifications N-glycosylated.
Function Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation.
Protein Name Hepatic And Glial Cell Adhesion Molecule
Glialcam
Hepatocyte Cell Adhesion Molecule
Protein Hepacam
Cellular Localisation Cytoplasm
Membrane
Single-Pass Type I Membrane Protein
Cytoplasmic Side
In Mcf-7 Breast Carcinoma And Hepatic Hep 3b2
1-7 And Hep-G2 Cell Lines
Localization Of Hepacam Is Cell Density-Dependent
In Well Spread Cells
Localized To Punctate Structures In The Perinuclear Membrane
And At Cell Surface Of Protusions
In Confluent Cells
Localized Predominantly To The Cytoplasmic Membrane
Particularly In Areas Of Cell-Cell Contacts
Colocalizes With Cdh1
Alternative Antibody Names Anti-Hepatic And Glial Cell Adhesion Molecule antibody
Anti-Glialcam antibody
Anti-Hepatocyte Cell Adhesion Molecule antibody
Anti-Protein Hepacam antibody
Anti-HEPACAM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance