Anti-HELQ antibody (1-270) (STJ114535)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal HEL308 (1-270) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:3000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HELQ |
| Gene ID | 113510 |
| Uniprot ID | HELQ_HUMAN |
| Immunogen Region | 1-270 |
| Immunogen Sequence | MDECGSRIRRRVSLPKRNRP SLGCIFGAPTAAELVPGDEG KEEEEMVAENRRRKTAGVLP VEVQPLLLSDSPECLVLGGG DTNPDLLRHMPTDRGVGDQP NDSEVDMFGDYDSFTENSFI AQVDDLEQKYMQLPEHKKHA TDFATENLCSESIKNKLSIT TIGNLTELQTDKHTENQSGY EGVTIEPGADLLYDVPSSQA IYFENLQNSSNDLGDHSMKE RDWKSSSHNTVNEELPHNC |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HEL308 (NP_598375.3). |
Additional Info
| Function | Single-stranded 3'-5' DNA helicase that plays a key role in homology-driven double-strand break (DSB) repair. Involved in different DSB repair mechanisms that are guided by annealing of extensive stretches of complementary bases at break ends, such as microhomology-mediated end-joining (MMEJ), single-strand annealing (SSA) or synthesis-dependent strand annealing (SDSA). Possesses both DNA unwinding and annealing activities. Forms a complex with RAD51, stimulating HELQ DNA helicase activity and ability to unwing DNA. Efficiently unwinds substrates containing 3' overhangs or a D-loop. In contrast, interaction with the replication protein A (RPA/RP-A) complex inhibits DNA unwinding by HELQ but strongly stimulates DNA strand annealing. Triggers displacement of RPA from single-stranded DNA to facilitate annealing of complementary sequences. |
| Protein Name | Helicase Polq-LikeMus308-Like HelicasePolq-Like Helicase |
| Database Links | |
| Cellular Localisation | NucleusChromosomeLocalizes To Sites Of Dna DamageLocalizes To Damaged Replication Forks |
| Alternative Antibody Names | Anti-Helicase Polq-Like antibodyAnti-Mus308-Like Helicase antibodyAnti-Polq-Like Helicase antibodyAnti-HELQ antibodyAnti-HEL308 antibody |
Information sourced from Uniprot.org