| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-HEL308 (1-270) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:3000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HELQ |
| Gene ID: |
113510 |
| Uniprot ID: |
HELQ_HUMAN |
| Immunogen Region: |
1-270 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HEL308 (NP_598375.3). |
| Immunogen Sequence: |
MDECGSRIRRRVSLPKRNRP SLGCIFGAPTAAELVPGDEG KEEEEMVAENRRRKTAGVLP VEVQPLLLSDSPECLVLGGG DTNPDLLRHMPTDRGVGDQP NDSEVDMFGDYDSFTENSFI AQVDDLEQKYMQLPEHKKHA TDFATENLCSESIKNKLSIT TIGNLTELQTDKHTENQSGY EGVTIEPGADLLYDVPSSQA IYFENLQNSSNDLGDHSMKE RDWKSSSHNTVNEELPHNC |
| Function | Single-stranded 3'-5' DNA helicase that plays a key role in homology-driven double-strand break (DSB) repair. Involved in different DSB repair mechanisms that are guided by annealing of extensive stretches of complementary bases at break ends, such as microhomology-mediated end-joining (MMEJ), single-strand annealing (SSA) or synthesis-dependent strand annealing (SDSA). Possesses both DNA unwinding and annealing activities. Forms a complex with RAD51, stimulating HELQ DNA helicase activity and ability to unwing DNA. Efficiently unwinds substrates containing 3' overhangs or a D-loop. In contrast, interaction with the replication protein A (RPA/RP-A) complex inhibits DNA unwinding by HELQ but strongly stimulates DNA strand annealing. Triggers displacement of RPA from single-stranded DNA to facilitate annealing of complementary sequences. |
| Protein Name | Helicase Polq-LikeMus308-Like HelicasePolq-Like Helicase |
| Cellular Localisation | NucleusChromosomeLocalizes To Sites Of Dna DamageLocalizes To Damaged Replication Forks |
| Alternative Antibody Names | Anti-Helicase Polq-Like antibodyAnti-Mus308-Like Helicase antibodyAnti-Polq-Like Helicase antibodyAnti-HELQ antibodyAnti-HEL308 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
