• Immunofluorescence analysis of U-2 OS cells using HEBP2 Rabbit polyclonal antibody (STJ118931) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using HEBP2 Rabbit polyclonal antibody (STJ118931) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using HEBP2 Rabbit polyclonal antibody (STJ118931) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of HEBP2 in paraffin-embedded Mouse kidney using HEBP2 Rabbit polyclonal antibody (STJ118931) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HEBP2 in paraffin-embedded Rat spleen using HEBP2 Rabbit polyclonal antibody (STJ118931) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HEBP2 Rabbit polyclonal antibody (STJ118931) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HEBP2 antibody (1-205) (STJ118931)

SKU:
STJ118931

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HEBP2 (1-205) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HEBP2
Gene ID: 23593
Uniprot ID: HEBP2_HUMAN
Immunogen Region: 1-205
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human HEBP2 (NP_055135.1).
Immunogen Sequence: MAEPLQPDPGAAEDAAAQAV ETPGWKAPEDAGPQPGSYEI RHYGPAKWVSTSVESMDWDS AIQTGFTKLNSYIQGKNEKE MKIKMTAPVTSYVEPGSGPF SESTITISLYIPSEQQFDPP RPLESDVFIEDRAEMTVFVR SFDGFSSAQKNQEQLLTLAS ILREDGKVFDEKVYYTAGYN SPVKLLNRNNEVWLIQKNEP TKENE
Tissue Specificity Detected in placenta.
Function Can promote mitochondrial permeability transition and facilitate necrotic cell death under different types of stress conditions.
Protein Name Heme-Binding Protein 2
Placental Protein 23
Pp23
Protein Soul
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasm
Mitochondrion
Mainly Localized To The Cytoplasm With A Much Lower Abundance In The Mitochondrion
Alternative Antibody Names Anti-Heme-Binding Protein 2 antibody
Anti-Placental Protein 23 antibody
Anti-Pp23 antibody
Anti-Protein Soul antibody
Anti-HEBP2 antibody
Anti-C6orf34 antibody
Anti-SOUL antibody

Information sourced from Uniprot.org

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