• Western blot analysis of extracts of mouse liver, using HBA2 antibody (STJ110725) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of Mouse placenta using HBA2 antibody (STJ110725) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Human placenta using HBA2 antibody (STJ110725) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Rat placenta using HBA2 antibody (STJ110725) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Rat placenta using HBA2 antibody (STJ110725) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Human placenta using HBA2 antibody (STJ110725) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse placenta using HBA2 antibody (STJ110725) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of mouse liver, using HBA2 antibody (STJ110725) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HBA2 antibody (1-142) (STJ110725)

SKU:
STJ110725

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HBA2 (1-142) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HBA1.HBA2
Gene ID: 3039/3040
Uniprot ID: HBA_HUMAN
Immunogen Region: 1-142
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human HBA2 (NP_000508.1).
Immunogen Sequence: MVLSPADKTNVKAAWGKVGA HAGEYGAEALERMFLSFPTT KTYFPHFDLSHGSAQVKGHG KKVADALTNAVAHVDDMPNA LSALSDLHAHKLRVDPVNFK LLSHCLLVTLAAHLPAEFTP AVHASLDKFLASVSTVLTSK YR
Tissue Specificity Red blood cells.
Post Translational Modifications The initiator Met is not cleaved in variant Thionville and is acetylated.
Function Involved in oxygen transport from the lung to the various peripheral tissues. Hemopressin: Hemopressin acts as an antagonist peptide of the cannabinoid receptor CNR1. Hemopressin-binding efficiently blocks cannabinoid receptor CNR1 and subsequent signaling.
Protein Name Hemoglobin Subunit Alpha
Alpha-Globin
Hemoglobin Alpha Chain Cleaved Into - Hemopressin
Database Links Reactome: R-HSA-1237044
Reactome: R-HSA-1247673
Reactome: R-HSA-2168880
Reactome: R-HSA-9707564
Reactome: R-HSA-9707616
Alternative Antibody Names Anti-Hemoglobin Subunit Alpha antibody
Anti-Alpha-Globin antibody
Anti-Hemoglobin Alpha Chain Cleaved Into - Hemopressin antibody
Anti-HBA1.HBA2 antibody

Information sourced from Uniprot.org

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