• Western blot analysis of extracts of various cell lines, using KAT1/HAT1 rabbit monoclonal antibody (STJ11102078) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of KAT1/HAT1 in paraffin-embedded human cervix cancer tissue using KAT1/HAT1 rabbit monoclonal antibody (STJ11102078) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of KAT1/HAT1 in paraffin-embedded human colon tissue using KAT1/HAT1 rabbit monoclonal antibody (STJ11102078) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of KAT1/HAT1 in paraffin-embedded mouse testis tissue using KAT1/HAT1 rabbit monoclonal antibody (STJ11102078) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of KAT1/HAT1 in paraffin-embedded rat colon tissue using KAT1/HAT1 rabbit monoclonal antibody (STJ11102078) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of C6 cells using KAT1/HAT1 rabbit monoclonal antibody (STJ11102078) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using KAT1/HAT1 rabbit monoclonal antibody (STJ11102078) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HAT1 antibody (200-400) [S8MR] (STJ11102078)

SKU:
STJ11102078

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-KAT1/HAT1 (200-400) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HAT1
Gene ID: 8520
Uniprot ID: HAT1_HUMAN
Immunogen Region: 200-400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-400 of human KAT1/HAT1 (NP_003633.2).
Immunogen Sequence: HYFLVFEKYNKDGATLFATV GYMTVYNYYVYPDKTRPRVS QMLILTPFQGQGHGAQLLET VHRYYTEFPTVLDITAEDPS KSYVKLRDFVLVKLCQDLPC FSREKLMQGFNEDMVIEAQQ KFKINKQHARRVYEILRLLV TDMSDAEQYRSYRLDIKRRL ISPYKKKQRDLAKMRKCLRP EELTNQMNQIEISMQHEQLE E
Post Translational Modifications Phosphorylated by AMPK at Ser-190.phosphorylation increases HAT1 activity.
Function Histone acetyltransferase that plays a role in different biological processes including cell cycle progression, glucose metabolism, histone production or DNA damage repair. Coordinates histone production and acetylation via H4 promoter binding. Acetylates histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, histone H2A at 'Lys-5' (H2AK5ac). Drives H4 production by chromatin binding to support chromatin replication and acetylation. Since transcription of H4 genes is tightly coupled to S-phase, plays an important role in S-phase entry and progression. Promotes homologous recombination in DNA repair by facilitating histone turnover and incorporation of acetylated H3.3 at sites of double-strand breaks. In addition, acetylates other substrates such as chromatin-related proteins. Acetylates also RSAD2 which mediates the interaction of ubiquitin ligase UBE4A with RSAD2 leading to RSAD2 ubiquitination and subsequent degradation. (Microbial infection) Contributes to hepatitis B virus (HBV) replication by acetylating histone H4 at the sites of 'Lys-5' and 'Lys-12' on the covalently closed circular DNA (cccDNA) minichromosome leading to its accumulation within the host cell.
Protein Name Histone Acetyltransferase Type B Catalytic Subunit
Histone Acetyltransferase 1
Database Links Reactome: R-HSA-3214847
Cellular Localisation Isoform A: Nucleus Matrix
Mitochondrion
Isoform B: Cytoplasm
Nucleus
Nucleus Matrix
Nucleoplasm
Localization Is Predominantly Nuclear In Normal Cells
Treatment With Hydrogen Peroxide Or Ionizing Radiation Enhances Nuclear Localization Through Redistribution Of Existing Protein
Alternative Antibody Names Anti-Histone Acetyltransferase Type B Catalytic Subunit antibody
Anti-Histone Acetyltransferase 1 antibody
Anti-HAT1 antibody
Anti-KAT1 antibody

Information sourced from Uniprot.org

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