• Western blot analysis of extracts of various cell lines, using HAS2 antibody (STJ111939) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates, using HAS2 Rabbit polyclonal antibody (STJ111939) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-HAS2 antibody (67-185) (STJ111939)

SKU:
STJ111939

€140.63 - €653.13
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HAS2 (67-185) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HAS2
Gene ID: 3037
Uniprot ID: HYAS2_HUMAN
Immunogen Region: 67-185
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 67-185 of human HAS2 (NP_005319.1).
Immunogen Sequence: EHRKMKKSLETPIKLNKTVA LCIAAYQEDPDYLRKCLQSV KRLTYPGIKVVMVIDGNSED DLYMMDIFSEVMGRDKSATY IWKNNFHEKGPGETDESHKE SSQHVTQLVLSNKSICIMQ
Tissue Specificity Expressed in fibroblasts.
Post Translational Modifications Phosphorylation at Thr-328 is essential for hyaluronan synthase activity. Phosphorylation at Thr-110 is required for transport from ER to Golgi. O-GlcNAcylation at Ser-221 increases the stability of HAS2 and plasma membrane localization. Ubiquitination at Lys-190.this ubiquitination is essential for hyaluronan synthase activity and homo- or hetero-oligomerization. Can also be poly-ubiquitinated. Deubiquitinated by USP17 and USP4. USP17 efficiently removes 'Lys-63'- and 'Lys-48'-linked polyubiquitin chains, whereas USP4 preferentially removes monoubiquitination and, partially, both 'Lys-63'- and 'Lys-48'-linked polyubiquitin chain.
Function Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer (Probable). Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of three isoenzymes responsible for cellular hyaluronan synthesis and it is particularly responsible for the synthesis of high molecular mass hyaluronan.
Protein Name Hyaluronan Synthase 2
Hyaluronate Synthase 2
Hyaluronic Acid Synthase 2
Ha Synthase 2
Database Links Reactome: R-HSA-2142850
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Endoplasmic Reticulum Membrane
Vesicle
Golgi Apparatus Membrane
Lysosome
Travels From Endoplasmic Reticulum (Er)
Golgi To Plasma Membrane And Either Back To Endosomes And Lysosomes
Or Out Into Extracellular Vesicles
Post-Translational Modifications Control Has2 Trafficking
Alternative Antibody Names Anti-Hyaluronan Synthase 2 antibody
Anti-Hyaluronate Synthase 2 antibody
Anti-Hyaluronic Acid Synthase 2 antibody
Anti-Ha Synthase 2 antibody
Anti-HAS2 antibody

Information sourced from Uniprot.org

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