| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-HAS2 (67-185) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HAS2 |
| Gene ID: |
3037 |
| Uniprot ID: |
HYAS2_HUMAN |
| Immunogen Region: |
67-185 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 67-185 of human HAS2 (NP_005319.1). |
| Immunogen Sequence: |
EHRKMKKSLETPIKLNKTVA LCIAAYQEDPDYLRKCLQSV KRLTYPGIKVVMVIDGNSED DLYMMDIFSEVMGRDKSATY IWKNNFHEKGPGETDESHKE SSQHVTQLVLSNKSICIMQ |
| Tissue Specificity | Expressed in fibroblasts. |
| Post Translational Modifications | Phosphorylation at Thr-328 is essential for hyaluronan synthase activity. Phosphorylation at Thr-110 is required for transport from ER to Golgi. O-GlcNAcylation at Ser-221 increases the stability of HAS2 and plasma membrane localization. Ubiquitination at Lys-190.this ubiquitination is essential for hyaluronan synthase activity and homo- or hetero-oligomerization. Can also be poly-ubiquitinated. Deubiquitinated by USP17 and USP4. USP17 efficiently removes 'Lys-63'- and 'Lys-48'-linked polyubiquitin chains, whereas USP4 preferentially removes monoubiquitination and, partially, both 'Lys-63'- and 'Lys-48'-linked polyubiquitin chain. |
| Function | Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer (Probable). Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of three isoenzymes responsible for cellular hyaluronan synthesis and it is particularly responsible for the synthesis of high molecular mass hyaluronan. |
| Protein Name | Hyaluronan Synthase 2Hyaluronate Synthase 2Hyaluronic Acid Synthase 2Ha Synthase 2 |
| Database Links | Reactome: R-HSA-2142850 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinEndoplasmic Reticulum MembraneVesicleGolgi Apparatus MembraneLysosomeTravels From Endoplasmic Reticulum (Er)Golgi To Plasma Membrane And Either Back To Endosomes And LysosomesOr Out Into Extracellular VesiclesPost-Translational Modifications Control Has2 Trafficking |
| Alternative Antibody Names | Anti-Hyaluronan Synthase 2 antibodyAnti-Hyaluronate Synthase 2 antibodyAnti-Hyaluronic Acid Synthase 2 antibodyAnti-Ha Synthase 2 antibodyAnti-HAS2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
