| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HAO1 |
| Gene ID: | 54363 |
| Uniprot ID: | HAOX1_HUMAN |
| Immunogen Region: | 1-370 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human HAO1/GOX (NP_060015.1). |
| Immunogen Sequence: | MLPRLICINDYEQHAKSVLP KSIYDYYRSGANDEETLADN IAAFSRWKLYPRMLRNVAET DLSTSVLGQRVSMPICVGAT AMQRMAHVDGELATVRACQS LGTGMMLSSWATSSIEEVAE AGPEALRWLQLYIYKDREVT KKLVRQAEKMGYKAIFVTVD TPYLGNRLDDVRNRFKLPPQ LRMKNFETSTLSFSPEENFG DDSGLAAYVAKAIDPSISWE DIKWLRRLTSLPIVAKGIL |
| Tissue Specificity | Highly expressed in liver. |
| Function | Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate. The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine.this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones. Can also catalyze the oxidation of glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, albeit with much lower catalytic efficiency. Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2. Is not active on L-lactate and 2-hydroxybutanoate. |
| Protein Name | 2-Hydroxyacid Oxidase 1 Haox1 Glycolate Oxidase Go Gox Glyoxylate Oxidase |
| Database Links | Reactome: R-HSA-389661 Reactome: R-HSA-9033241 |
| Cellular Localisation | Peroxisome Matrix |
| Alternative Antibody Names | Anti-2-Hydroxyacid Oxidase 1 antibody Anti-Haox1 antibody Anti-Glycolate Oxidase antibody Anti-Go antibody Anti-Gox antibody Anti-Glyoxylate Oxidase antibody Anti-HAO1 antibody Anti-GOX1 antibody |
Information sourced from Uniprot.org