• Dot-blot analysis of all sorts of peptides using Lactic acid-Histone H3-K18 antibody (STJ11101498) at 1:1000 dilution.
  • Western blot analysis of lysates from MCF7 cells, using Lactic acid-Histone H3-K18 Rabbit polyclonal antibody (STJ11101498) at 1:500 dilution. MCF7 cells were treated by Sodium lactate (100mM) for 24h. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5min.

Anti-H3K18la antibody (STJ11101498)

SKU:
STJ11101498

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Lactic acid-Histone H3-K18 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: DB 1:500-1:4000
WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic lactylated peptide around K18 of human Histone H3 (NP_003520.1).
Immunogen Sequence: APRKQ
Background The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.

Information sourced from Uniprot.org

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