• Immunofluorescence analysis of U2OS cells using GZMA antibody (STJ27987) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using GZMA Rabbit polyclonal antibody (STJ27987) at 1:2500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GZMA antibody (29-262) (STJ27987)

SKU:
STJ27987

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GZMA (29-262) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GZMA
Gene ID: 3001
Uniprot ID: GRAA_HUMAN
Immunogen Region: 29-262
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-262 of human GZMA (NP_006135.2).
Immunogen Sequence: IIGGNEVTPHSRPYMVLLSL DRKTICAGALIAKDWVLTAA HCNLNKRSQVILGAHSITRE EPTKQIMLVKKEFPYPCYDP ATREGDLKLLQLMEKAKINK YVTILHLPKKGDDVKPGTMC QVAGWGRTHNSASWSDTLRE VNITIIDRKVCNDRNHYNFN PVIGMNMVCAGSLRGGRDSC NGDSGSPLLCEGVFRGVTSF GLENKCGDPRGPGVYILLSK KHLNWIIMTIKGAV
Function Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-B (GSDMB), releasing the pore-forming moiety of GSDMB, thereby triggering pyroptosis and target cell death. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA.
Protein Name Granzyme A
Ctl Tryptase
Cytotoxic T-Lymphocyte Proteinase 1
Fragmentin-1
Granzyme-1
Hanukkah Factor
H Factor
Hf
Cellular Localisation Isoform Alpha: Secreted
Cytoplasmic Granule
Delivered Into The Target Cell By Perforin
Alternative Antibody Names Anti-Granzyme A antibody
Anti-Ctl Tryptase antibody
Anti-Cytotoxic T-Lymphocyte Proteinase 1 antibody
Anti-Fragmentin-1 antibody
Anti-Granzyme-1 antibody
Anti-Hanukkah Factor antibody
Anti-H Factor antibody
Anti-Hf antibody
Anti-GZMA antibody
Anti-CTLA3 antibody
Anti-HFSP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance