Anti-GTF2H1 antibody (1-425) (STJ28897)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GTF2H1 (1-425) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GTF2H1 |
| Gene ID | 2965 |
| Uniprot ID | TF2H1_HUMAN |
| Immunogen Region | 1-425 |
| Immunogen Sequence | MATSSEEVLLIVKKVRQKKQ DGALYLMAERIAWAPEGKDR FTISHMYADIKCQKISPEGK AKIQLQLVLHAGDTTNFHFS NESTAVKERDAVKDLLQQLL PKFKRKANKELEEKNRMLQE DPVLFQLYKDLVVSQVISAE EFWANRLNVNATDSSSTSNH KQDVGISAAFLADVRPQTDG CNGLRYNLTSDIIESIFRTY PAVKMKYAENVPHNMTEKEF WTRFFQSHYFHRDRLNTGS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-425 of human GTF2H1 (NP_001135779.1). |
Additional Info
| Post Translational Modifications | (Microbial infection) Upon Rift valley fever virus infection, interacts with NSs leading to ubiquitination by the NSs-FBXO3 E3 ligase and proteasome-dependent degradation. |
| Function | Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. |
| Protein Name | General Transcription Factor Iih Subunit 1Basic Transcription Factor 2 62 Kda SubunitBtf2 P62General Transcription Factor Iih Polypeptide 1Tfiih Basal Transcription Factor Complex P62 Subunit |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167160Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-167200Reactome: R-HSA-167246Reactome: R-HSA-427413Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-674695Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6796648Reactome: R-HSA-72086Reactome: R-HSA-73762Reactome: R-HSA-73772Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-73863Reactome: R-HSA-75953Reactome: R-HSA-75955Reactome: R-HSA-76042Reactome: R-HSA-77075 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-General Transcription Factor Iih Subunit 1 antibodyAnti-Basic Transcription Factor 2 62 Kda Subunit antibodyAnti-Btf2 P62 antibodyAnti-General Transcription Factor Iih Polypeptide 1 antibodyAnti-Tfiih Basal Transcription Factor Complex P62 Subunit antibodyAnti-GTF2H1 antibodyAnti-BTF2 antibody |
Information sourced from Uniprot.org