| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GSPT1 |
| Gene ID: | 2935 |
| Uniprot ID: | ERF3A_HUMAN |
| Immunogen Region: | 138-420 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 138-420 of human GSPT1 (NP_001123478.1). |
| Immunogen Sequence: | SMELSEPIENGETEMSPEES WEHKEEISEAEPGGGSLGDG RPPEESAHEMMEEEEEIPKP KSVVAPPGAPKKEHVNVVFI GHVDAGKSTIGGQIMYLTGM VDKRTLEKYEREAKEKNRET WYLSWALDTNQEERDKGKTV EVGRAYFETEKKHFTILDAP GHKSFVPNMIGGASQADLAV LVISARKGEFETGFEKGGQT REHAMLAKTAGVKHLIVLIN KMDDPTVNWSNERYEECKE |
| Function | GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA. GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes. |
| Protein Name | Eukaryotic Peptide Chain Release Factor Gtp-Binding Subunit Erf3a Eukaryotic Peptide Chain Release Factor Subunit 3a Erf3a G1 To S Phase Transition Protein 1 Homolog |
| Database Links | Reactome: R-HSA-72764 Reactome: R-HSA-9010553 Reactome: R-HSA-975956 Reactome: R-HSA-975957 |
| Alternative Antibody Names | Anti-Eukaryotic Peptide Chain Release Factor Gtp-Binding Subunit Erf3a antibody Anti-Eukaryotic Peptide Chain Release Factor Subunit 3a antibody Anti-Erf3a antibody Anti-G1 To S Phase Transition Protein 1 Homolog antibody Anti-GSPT1 antibody Anti-ERF3A antibody |
Information sourced from Uniprot.org