![Western blot analysis of lysates from wild type (WT) and Gelsolin Rabbit polyclonal antibody knockdown (KD) HeLa cells, using [KD Validated] Gelsolin Rabbit polyclonal antibody (STJ11104659) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177303/368966/STJ11104659_3__79905.1713134443.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Gelsolin Rabbit polyclonal antibody knockdown (KD) HeLa cells, using [KD Validated] Gelsolin Rabbit polyclonal antibody (STJ11104659) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western blot analysis of lysates from C2C12 cells, using [KD Validated] Gelsolin Rabbit polyclonal antibody (STJ11104659) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177303/368967/STJ11104659_4__04596.1713134443.jpg?c=1 "Western blot analysis of lysates from C2C12 cells, using [KD Validated] Gelsolin Rabbit polyclonal antibody (STJ11104659) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.")
![Western blot analysis of lysates from HepG2 cells, using [KD Validated] Gelsolin Rabbit polyclonal antibody (STJ11104659) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177303/368968/STJ11104659_5__38919.1713134444.jpg?c=1 "Western blot analysis of lysates from HepG2 cells, using [KD Validated] Gelsolin Rabbit polyclonal antibody (STJ11104659) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
| Applications: | WB/IHC-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:100-1:500IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GSN |
| Gene ID: | 2934 |
| Uniprot ID: | GELS_HUMAN |
| Immunogen Region: | 28-782 |
| Immunogen Sequence: | ATASRGASQAGAPQGRVPEA RPNSMVVEHPEFLKAGKEPG LQIWRVEKFDLVPVPTNLYG DFFTGDAYVILKTVQLRNGN LQYDLHYWLGNECSQDESGA AAIFTVQLDDYLNGRAVQHR EVQGFESATFLGYFKSGLKY KKGGVASGFKHVVPNEVVVQ RLFQVKGRRVVRATEVPVSW ESFNNGDCFILDLGNNIHQW CGSNSNRYERLKATQVSKGI RDNERSGRARVHVSEEGTE |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-782 of human Gelsolin (NP_000168.1). |
| Tissue Specificity | Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells. |
| Post Translational Modifications | Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids. |
| Function | Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. |
| Protein Name | GelsolinAgelActin-Depolymerizing FactorAdfBrevin |
| Database Links | Reactome: R-HSA-264870Reactome: R-HSA-6798695Reactome: R-HSA-9662361Reactome: R-HSA-977225 |
| Cellular Localisation | Isoform 2: CytoplasmCytoskeletonIsoform 1: Secreted |
| Alternative Antibody Names | Anti-Gelsolin antibodyAnti-Agel antibodyAnti-Actin-Depolymerizing Factor antibodyAnti-Adf antibodyAnti-Brevin antibodyAnti-GSN antibody |
Information sourced from Uniprot.org
