• Immunohistochemistry analysis of Gelsolin in paraffin-embedded human tonsil using Gelsolin Rabbit polyclonal antibody (STJ11104659) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Gelsolin in paraffin-embedded human liver using Gelsolin Rabbit polyclonal antibody (STJ11104659) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and Gelsolin Rabbit polyclonal antibody knockdown (KD) HeLa cells, using [KD Validated] Gelsolin Rabbit polyclonal antibody (STJ11104659) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of lysates from C2C12 cells, using [KD Validated] Gelsolin Rabbit polyclonal antibody (STJ11104659) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Western blot analysis of lysates from HepG2 cells, using [KD Validated] Gelsolin Rabbit polyclonal antibody (STJ11104659) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GSN antibody (28-782) (STJ11104659)

SKU:
STJ11104659

£134.50 - £666.50
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Gelsolin (28-782) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GSN
Gene ID: 2934
Uniprot ID: GELS_HUMAN
Immunogen Region: 28-782
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-782 of human Gelsolin (NP_000168.1).
Immunogen Sequence: ATASRGASQAGAPQGRVPEA RPNSMVVEHPEFLKAGKEPG LQIWRVEKFDLVPVPTNLYG DFFTGDAYVILKTVQLRNGN LQYDLHYWLGNECSQDESGA AAIFTVQLDDYLNGRAVQHR EVQGFESATFLGYFKSGLKY KKGGVASGFKHVVPNEVVVQ RLFQVKGRRVVRATEVPVSW ESFNNGDCFILDLGNNIHQW CGSNSNRYERLKATQVSKGI RDNERSGRARVHVSEEGTE
Tissue Specificity Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
Post Translational Modifications Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
Function Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Protein Name Gelsolin
Agel
Actin-Depolymerizing Factor
Adf
Brevin
Database Links Reactome: R-HSA-264870
Reactome: R-HSA-6798695
Reactome: R-HSA-9662361
Reactome: R-HSA-977225
Cellular Localisation Isoform 2: Cytoplasm
Cytoskeleton
Isoform 1: Secreted
Alternative Antibody Names Anti-Gelsolin antibody
Anti-Agel antibody
Anti-Actin-Depolymerizing Factor antibody
Anti-Adf antibody
Anti-Brevin antibody
Anti-GSN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance