• Western blot analysis of lysates from 293 cells, treated with TNF-a 20ng/ml 30', using GSK3 alpha/beta Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of 293 cells using GSK3 Alpha/Beta Polyclonal Antibody diluted at 1：1000
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using GSK3 alpha/beta Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using GSK3 Alpha/Beta Polyclonal Antibody diluted at 1：1000

Anti-GSK3 Alpha/Beta antibody (246-295 aa) (STJ93446)

SKU:
STJ93446

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/D.melanogaster
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Glycogen synthase kinase-3 alpha and Glycogen synthase kinase-3 beta (246-295 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GSK3B
GSK3A
Gene ID: 2932
2931
Uniprot ID: GSK3B_HUMAN
GSK3A_HUMAN
Immunogen Region: 246-295 aa
Specificity: GSK3 Alpha/Beta Polyclonal Antibody detects endogenous levels of GSK3 Alpha/Beta protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human GSK3 alpha/beta at the amino acid range 246-295
Function Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation. Acts as a regulator of autophagy by mediating phosphorylation of KAT5/TIP60 under starvation conditions, leading to activate KAT5/TIP60 acetyltransferase activity and promote acetylation of key autophagy regulators, such as ULK1 and RUBCNL/Pacer. Negatively regulates extrinsic apoptotic signaling pathway via death domain receptors. Promotes the formation of an anti-apoptotic complex, made of DDX3X, BRIC2 and GSK3B, at death receptors, including TNFRSF10B. The anti-apoptotic function is most effective with weak apoptotic signals and can be overcome by stronger stimulation.
Protein Name Glycogen Synthase Kinase-3 Alpha
Gsk-3 Alpha
Serine/Threonine-Protein Kinase Gsk3a
Database Links Reactome: R-HSA-198323
Reactome: R-HSA-381038
Reactome: R-HSA-5674400
Reactome: R-HSA-9635465
Reactome: R-HSA-9682706
Reactome: R-HSA-9694686
Alternative Antibody Names Anti-Glycogen Synthase Kinase-3 Alpha antibody
Anti-Gsk-3 Alpha antibody
Anti-Serine/Threonine-Protein Kinase Gsk3a antibody
Anti-GSK3A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance