• Immunofluorescence analysis of L929 cells using GRSF1 Rabbit polyclonal antibody (STJ11100184) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GRSF1 Rabbit polyclonal antibody (STJ11100184) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of GRSF1 in paraffin-embedded Human esophageal cancer using GRSF1 Rabbit polyclonal antibody (STJ11100184) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GRSF1 in paraffin-embedded Rat liver using GRSF1 Rabbit polyclonal antibody (STJ11100184) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GRSF1 in paraffin-embedded Mouse brain using GRSF1 Rabbit polyclonal antibody (STJ11100184) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using GRSF1 Rabbit polyclonal antibody (STJ11100184) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GRSF1 antibody (100-200) (STJ11100184)

SKU:
STJ11100184

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GRSF1 (100-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRSF1
Gene ID: 2926
Uniprot ID: GRSF1_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human GRSF1 (NP_002083.4).
Immunogen Sequence: SLLPQSLAAAAAVPTRSYSQ ESKTTYLEDLPPPPEYELAP SKLEEEVDDVFLIRAQGLPW SCTMEDVLNFFSDCRIRNGE NGIHFLLNRDGKRRGDALIE M
Function Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences. Involved in the degradosome-mediated decay of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Acts by unwinding G-quadruplex RNA structures in MT-ncRNA, thus facilitating their degradation by the degradosome. G-quadruplexes (G4) are non-canonical 4 stranded structures formed by transcripts from the light strand of mtDNA.
Protein Name G-Rich Sequence Factor 1
Grsf-1
Database Links Reactome: R-HSA-192823
Cellular Localisation Isoform 1: Mitochondrion Matrix
Localizes To Mitochondrial Rna Granules Found In Close Proximity To The Mitochondrial Nucleoids
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-G-Rich Sequence Factor 1 antibody
Anti-Grsf-1 antibody
Anti-GRSF1 antibody

Information sourced from Uniprot.org

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