Anti-GRSF1 antibody (100-200) (STJ11100184)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GRSF1 (100-200) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GRSF1 |
| Gene ID | 2926 |
| Uniprot ID | GRSF1_HUMAN |
| Immunogen Region | 100-200 |
| Immunogen Sequence | SLLPQSLAAAAAVPTRSYSQ ESKTTYLEDLPPPPEYELAP SKLEEEVDDVFLIRAQGLPW SCTMEDVLNFFSDCRIRNGE NGIHFLLNRDGKRRGDALIE M |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human GRSF1 (NP_002083.4). |
Additional Info
| Function | Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences. Involved in the degradosome-mediated decay of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Acts by unwinding G-quadruplex RNA structures in MT-ncRNA, thus facilitating their degradation by the degradosome. G-quadruplexes (G4) are non-canonical 4 stranded structures formed by transcripts from the light strand of mtDNA. |
| Protein Name | G-Rich Sequence Factor 1Grsf-1 |
| Database Links | Reactome: R-HSA-192823Reactome: R-HSA-9836573 |
| Cellular Localisation | Isoform 1: Mitochondrion MatrixLocalizes To Mitochondrial Rna Granules Found In Close Proximity To The Mitochondrial NucleoidsIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-G-Rich Sequence Factor 1 antibodyAnti-Grsf-1 antibodyAnti-GRSF1 antibody |
Information sourced from Uniprot.org