| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GRSF1 |
| Gene ID: | 2926 |
| Uniprot ID: | GRSF1_HUMAN |
| Immunogen Region: | 100-200 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human GRSF1 (NP_002083.4). |
| Immunogen Sequence: | SLLPQSLAAAAAVPTRSYSQ ESKTTYLEDLPPPPEYELAP SKLEEEVDDVFLIRAQGLPW SCTMEDVLNFFSDCRIRNGE NGIHFLLNRDGKRRGDALIE M |
| Function | Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences. Involved in the degradosome-mediated decay of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Acts by unwinding G-quadruplex RNA structures in MT-ncRNA, thus facilitating their degradation by the degradosome. G-quadruplexes (G4) are non-canonical 4 stranded structures formed by transcripts from the light strand of mtDNA. |
| Protein Name | G-Rich Sequence Factor 1 Grsf-1 |
| Database Links | Reactome: R-HSA-192823 Reactome: R-HSA-9836573 |
| Cellular Localisation | Isoform 1: Mitochondrion Matrix Localizes To Mitochondrial Rna Granules Found In Close Proximity To The Mitochondrial Nucleoids Isoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-G-Rich Sequence Factor 1 antibody Anti-Grsf-1 antibody Anti-GRSF1 antibody |
Information sourced from Uniprot.org