• Western blot analysis of extracts of various cell lines, using GRK6 antibody (STJ28462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GRK6 antibody (268-465) (STJ28462)

SKU:
STJ28462

€140.63 - €653.13
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GRK6 (268-465) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRK6
Gene ID: 2870
Uniprot ID: GRK6_HUMAN
Immunogen Region: 268-465
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 268-465 of human GRK6 (NP_001004106.1).
Immunogen Sequence: GGDLKFHIYHMGQAGFPEAR AVFYAAEICCGLEDLHRERI VYRDLKPENILLDDHGHIRI SDLGLAVHVPEGQTIKGRVG TVGYMAPEVVKNERYTFSPD WWALGCLLYEMIAGQSPFQQ RKKKIKREEVERLVKEVPEE YSERFSPQARSLCSQLLCKD PAERLGCRGGSAREVKEHPL FKKLNFKRLGAGMLEPPF
Tissue Specificity Widely expressed.
Post Translational Modifications It is uncertain whether palmitoylation is on Cys-561 and/or Cys-562 and/or Cys-565.
Function Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).
Protein Name G Protein-Coupled Receptor Kinase 6
G Protein-Coupled Receptor Kinase Grk6
Database Links Reactome: R-HSA-418555
Cellular Localisation Membrane
Lipid-Anchor
Alternative Antibody Names Anti-G Protein-Coupled Receptor Kinase 6 antibody
Anti-G Protein-Coupled Receptor Kinase Grk6 antibody
Anti-GRK6 antibody
Anti-GPRK6 antibody

Information sourced from Uniprot.org

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