• Western blot analysis of lysates from NIH/3T3 cells, treated with Insulin 0.01U/ml 15', using GRB10 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GRB10 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of 3T3 cells using GRB10 Polyclonal Antibody diluted at 1：2000
  • Immunofluorescence analysis of HepG2 cells, using GRB10 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using GRB10 Polyclonal Antibody diluted at 1：2000

Anti-GRB10 antibody (33-82 aa) (STJ93419)

SKU:
STJ93419

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Growth factor receptor-bound protein 10 (33-82 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRB10
Gene ID: 2887
Uniprot ID: GRB10_HUMAN
Immunogen Region: 33-82 aa
Specificity: GRB10 Polyclonal Antibody detects endogenous levels of GRB10 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human GRB10 at the amino acid range 33-82
Post Translational Modifications Phosphorylated on serine residues upon EGF, FGF and PDGF stimulation. Phosphorylated at Tyr-67 by TEC.
Function Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
Protein Name Growth Factor Receptor-Bound Protein 10
Grb10 Adapter Protein
Insulin Receptor-Binding Protein Grb-Ir
Database Links Reactome: R-HSA-1433557
Reactome: R-HSA-74713
Reactome: R-HSA-74749
Reactome: R-HSA-74751
Reactome: R-HSA-8853659
Reactome: R-HSA-9607240
Reactome: R-HSA-9648895
Cellular Localisation Cytoplasm
When Complexed With Nedd4 And Igf1r
Follows Igf1r Internalization
Remaining Associated With Early Endosomes
Uncouples From Igf1r-Containing Endosomes Before The Sorting Of The Receptor To The Lysosomal Compartment
Alternative Antibody Names Anti-Growth Factor Receptor-Bound Protein 10 antibody
Anti-Grb10 Adapter Protein antibody
Anti-Insulin Receptor-Binding Protein Grb-Ir antibody
Anti-GRB10 antibody
Anti-GRBIR antibody
Anti-KIAA0207 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance