Anti-GPR15 antibody (201-250 aa) (STJ93343)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human GPR15 at the amino acid range 201-250
General Information
| Short Description | Rabbit polyclonal anti-G-protein coupled receptor 15 (201-250 aa) for use in WB, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IF 1:200-1:1000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GPR15 |
| Gene ID | 2838 |
| Uniprot ID | GPR15_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human GPR15 at the amino acid range 201-250 |
| Immunogen Region | 201-250 aa |
| Specificity | GPR15 Polyclonal Antibody detects endogenous levels of GPR15 protein. |
Additional Info
| Post Translational Modifications | Phosphorylation is necessary for YWHAE binding and efficient surface expression. O-glycosylated. Sialylated O-glycans in the N-terminal tail inhibits binding of GPR15LG. Sulfation is required for efficient binding of GPR15LG. |
| Function | G protein-coupled receptor that plays an important role in immune homeostasis. Acts via its natural ligand GPR15LG, a chemokine-like polypeptide strongly expressed in gastrointestinal tissues. GPR15-GPR15LG signaling axis regulates intestinal homeostasis and inflammation through the migration of immune cells. Controls thereby the specific homing of T-cells, particularly FOXP3+ regulatory T-cells (Tregs), to the large intestine lamina propria. Also required for skin localization of thymus-derived dendritic epidermal T-cells. Plays an important role in mediating cytoprotective function as well as angiogenesis of thrombomodulin. Mechanistically, preferentially signals through the Gi/o pathway to inhibit adenylate cyclase activity and activate a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores. (Microbial infection) Acts as an alternative coreceptor with CD4 for HIV-1 infection. |
| Protein Name | G-Protein Coupled Receptor 15Brother Of BonzoBob |
| Database Links | Reactome: R-HSA-418555 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-G-Protein Coupled Receptor 15 antibodyAnti-Brother Of Bonzo antibodyAnti-Bob antibodyAnti-GPR15 antibody |
Information sourced from Uniprot.org