• Immunofluorescence analysis of HepG2 cells using GPLD1 Rabbit polyclonal antibody (STJ28695) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A-549 cells using GPLD1 Rabbit polyclonal antibody (STJ28695) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using GPLD1 Rabbit polyclonal antibody (STJ28695) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using GPLD1 Rabbit polyclonal antibody (STJ28695) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using GPLD1 antibody (STJ28695) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GPLD1 antibody (24-160) (STJ28695)

SKU:
STJ28695

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GPLD1 (24-160) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPLD1
Gene ID: 2822
Uniprot ID: PHLD_HUMAN
Immunogen Region: 24-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human GPLD1 (NP_001494.2).
Immunogen Sequence: CGLSTHVEIGHRALEFLQLH NGRVNYRELLLEHQDAYQAG IVFPDCFYPSICKGGKFHDV SESTHWTPFLNASVHYIREN YPLPWEKDTEKLVAFLFGIT SHMAADVSWHSLGLEQGFLR TMGAIDFHGSYSEAHSA
Function This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
Protein Name Phosphatidylinositol-Glycan-Specific Phospholipase D
Pi-G Pld
Glycoprotein Phospholipase D
Glycosyl-Phosphatidylinositol-Specific Phospholipase D
Gpi-Pld
Gpi-Specific Phospholipase D
Database Links Reactome: R-HSA-163125
Cellular Localisation Secreted
Alternative Antibody Names Anti-Phosphatidylinositol-Glycan-Specific Phospholipase D antibody
Anti-Pi-G Pld antibody
Anti-Glycoprotein Phospholipase D antibody
Anti-Glycosyl-Phosphatidylinositol-Specific Phospholipase D antibody
Anti-Gpi-Pld antibody
Anti-Gpi-Specific Phospholipase D antibody
Anti-GPLD1 antibody
Anti-PIGPLD1 antibody

Information sourced from Uniprot.org

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