• Western blot analysis of extracts of U-251MG cells, using GP2 antibody (STJ28461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GP2 antibody (111-387) (STJ28461)

SKU:
STJ28461

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GP2 (111-387) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GP2
Gene ID: 2813
Uniprot ID: GP2_HUMAN
Immunogen Region: 111-387
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 111-387 of human GP2 (NP_001007243.2).
Immunogen Sequence: CSSILQTEERNWVSVTSPVQ ASACRNILERNQTHAIYKNT LSLVNDFIIRDTILNINFQC AYPLDMKVSLQAALQPIVSS LNVSVDGNGEFIVRMALFQD QNYTNPYEGDAVELSVESVL YVGAILEQGDTSRFNLVLRN CYATPTEDKADLVKYFIIRN SCSNQRDSTIHVEENGQSSE SRFSVQMFMFAGHYDLVFLH CEIHLCDSLNEQCQPSCSRS QVRSEVPAIDLARVLDLGP
Tissue Specificity Expressed in pancreas (at protein level). Specifically expressed by M (microfold) cells which are atypical epithelial cells of the intestine (at protein level).
Post Translational Modifications N-glycosylated. Glycosylated Asn-65 may be required for interaction with bacterial adhesin fimH.
Function Functions as an intestinal M-cell transcytotic receptor specific for type-I-piliated bacteria that participates in the mucosal immune response toward these bacteria. At the apical membrane of M-cells it binds fimH, a protein of the bacteria type I pilus tip. Internalizes bound bacteria, like E.coli and S.typhimurium, from the lumen of the intestine and delivers them, through M-cells, to the underlying organized lymphoid follicles where they are captured by antigen-presenting dendritic cells to elicit a mucosal immune response.
Protein Name Pancreatic Secretory Granule Membrane Major Glycoprotein Gp2
Pancreatic Zymogen Granule Membrane Protein Gp-2
Zap75
Database Links Reactome: R-HSA-163125
Cellular Localisation Zymogen Granule Membrane
Lipid-Anchor
Gpi-Anchor
Secreted
Cell Membrane
Apical Cell Membrane
Membrane Raft
Endosome
After Cleavage
In The Pancreatic Juice
Alternative Antibody Names Anti-Pancreatic Secretory Granule Membrane Major Glycoprotein Gp2 antibody
Anti-Pancreatic Zymogen Granule Membrane Protein Gp-2 antibody
Anti-Zap75 antibody
Anti-GP2 antibody

Information sourced from Uniprot.org

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