• Immunofluorescence analysis of NIH-3T3 cells using PIST/GOPC Rabbit monoclonal antibody (STJ11101244) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PIST/GOPC Rabbit monoclonal antibody (STJ11101244) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PIST/GOPC in paraffin-embedded human thyroid cancer using PIST/GOPC Rabbit monoclonal antibody (STJ11101244) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse lung, using PIST/GOPC Rabbit monoclonal antibody (STJ11101244) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of various lysates using PIST/GOPC Rabbit monoclonal antibody (STJ11101244) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GOPC antibody (1-100) [S4MR] (STJ11101244)

SKU:
STJ11101244

$197.78 - $863.33
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PIST (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GOPC
Gene ID: 57120
Uniprot ID: GOPC_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PIST/GOPC (Q9HD26).
Immunogen Sequence: MSAGGPCPAAAGGGPGGASC SVGAPGGVSMFRWLEVLEKE FDKAFVDVDLLLGEIDPDQA DITYEGRQKMTSLSSCFAQL CHKAQSVSQINHKLEAQLVD
Tissue Specificity Ubiquitously expressed.
Function Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Together with MARCHF2 mediates the ubiquitination and lysosomal degradation of CFTR. Overexpression results in CFTR intracellular retention and lysosomaldegradation in the lysosomes.
Protein Name Golgi-Associated Pdz And Coiled-Coil Motif-Containing Protein
Cftr-Associated Ligand
Fused In Glioblastoma
Pdz Protein Interacting Specifically With Tc10
Pist
Database Links Reactome: R-HSA-5627083
Reactome: R-HSA-9013406
Cellular Localisation Cytoplasm
Golgi Apparatus Membrane
Peripheral Membrane Protein
Golgi Apparatus
Trans-Golgi Network Membrane
Synapse
Postsynaptic Density
Cell Projection
Dendrite
Enriched In Synaptosomal And Postsynaptic Densities (Psd) Fractions
Expressed In Cell Bodies And Dendrites Of Purkinje Cells
Localized At The Trans-Golgi Network (Tgn) Of Spermatids And The Medulla Of Round Spermatides
Alternative Antibody Names Anti-Golgi-Associated Pdz And Coiled-Coil Motif-Containing Protein antibody
Anti-Cftr-Associated Ligand antibody
Anti-Fused In Glioblastoma antibody
Anti-Pdz Protein Interacting Specifically With Tc10 antibody
Anti-Pist antibody
Anti-GOPC antibody
Anti-CAL antibody
Anti-FIG antibody

Information sourced from Uniprot.org

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