| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-GOLGA5 (1-300) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GOLGA5 |
| Gene ID: |
9950 |
| Uniprot ID: |
GOGA5_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GOLGA5 (NP_005104.3). |
| Immunogen Sequence: |
MSWFVDLAGKAEDLLNRVDQ GAATALSRKDNASNIYSKNT DYTELHQQNTDLIYQTGPKS TYISSAADNIRNQKATILAG TANVKVGSRTPVEASHPVEN ASVPRPSSHFVRRKKSEPDD ELLFDFLNSSQKEPTGRVEI RKEKGKTPVFQSSQTSSVSS VNPSVTTIKTIEENSFGSQT HEAASNSDSSHEGQEESSKE NVSSNAACPDHTPTPNDDGK SHELSNLRLENQLLRNEVQ |
| Tissue Specificity | Ubiquitous. Highly expressed in seminiferous tubules and Leydig cells in testis, and detected at much lower levels in the other tissues tested. Expression is very low or not detectable in spermatozoa. |
| Post Translational Modifications | Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase. |
| Function | Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport. |
| Protein Name | Golgin Subfamily A Member 5Cell Proliferation-Inducing Gene 31 ProteinGolgin-84Protein Ret-IiRet-Fused Gene 5 Protein |
| Database Links | Reactome: R-HSA-6811438 |
| Cellular Localisation | Golgi Apparatus MembraneSingle-Pass Type Iv Membrane ProteinFound Throughout The GolgiBoth On Cisternae AndAt Higher AbundanceOn The Tubulo-Vesicular Structures Of The Cis-Golgi Network |
| Alternative Antibody Names | Anti-Golgin Subfamily A Member 5 antibodyAnti-Cell Proliferation-Inducing Gene 31 Protein antibodyAnti-Golgin-84 antibodyAnti-Protein Ret-Ii antibodyAnti-Ret-Fused Gene 5 Protein antibodyAnti-GOLGA5 antibodyAnti-RETII antibodyAnti-RFG5 antibodyAnti-PIG31 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
