• Immunofluorescence analysis of L929 cells using GOLGA5 Rabbit polyclonal antibody (STJ118213) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GOLGA5 Rabbit polyclonal antibody (STJ118213) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using GOLGA5 Rabbit polyclonal antibody (STJ118213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GOLGA5 antibody (1-300) (STJ118213)

SKU:
STJ118213

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GOLGA5
Gene ID: 9950
Uniprot ID: GOGA5_HUMAN
Immunogen Region: 1-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GOLGA5 (NP_005104.3).
Immunogen Sequence: MSWFVDLAGKAEDLLNRVDQ GAATALSRKDNASNIYSKNT DYTELHQQNTDLIYQTGPKS TYISSAADNIRNQKATILAG TANVKVGSRTPVEASHPVEN ASVPRPSSHFVRRKKSEPDD ELLFDFLNSSQKEPTGRVEI RKEKGKTPVFQSSQTSSVSS VNPSVTTIKTIEENSFGSQT HEAASNSDSSHEGQEESSKE NVSSNAACPDHTPTPNDDGK SHELSNLRLENQLLRNEVQ
Tissue Specificity Ubiquitous. Highly expressed in seminiferous tubules and Leydig cells in testis, and detected at much lower levels in the other tissues tested. Expression is very low or not detectable in spermatozoa.
Post Translational Modifications Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase.
Function Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.
Protein Name Golgin Subfamily A Member 5
Cell Proliferation-Inducing Gene 31 Protein
Golgin-84
Protein Ret-Ii
Ret-Fused Gene 5 Protein
Database Links Reactome: R-HSA-6811438
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Iv Membrane Protein
Found Throughout The Golgi
Both On Cisternae And
At Higher Abundance
On The Tubulo-Vesicular Structures Of The Cis-Golgi Network
Alternative Antibody Names Anti-Golgin Subfamily A Member 5 antibody
Anti-Cell Proliferation-Inducing Gene 31 Protein antibody
Anti-Golgin-84 antibody
Anti-Protein Ret-Ii antibody
Anti-Ret-Fused Gene 5 Protein antibody
Anti-GOLGA5 antibody
Anti-RETII antibody
Anti-RFG5 antibody
Anti-PIG31 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance