• Immunofluorescence analysis of L929 cells using GOLGA4 Rabbit polyclonal antibody (STJ112254) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using GOLGA4 Rabbit polyclonal antibody (STJ112254) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GOLGA4 Rabbit polyclonal antibody (STJ112254) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using GOLGA4 antibody (STJ112254) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GOLGA4 antibody (1-150) (STJ112254)

SKU:
STJ112254

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GOLGA4 (1-150) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GOLGA4
Gene ID: 2803
Uniprot ID: GOGA4_HUMAN
Immunogen Region: 1-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GOLGA4 (NP_002069.2).
Immunogen Sequence: MFKKLKQKISEEQQQLQQAL APAQASSNSSTPTRMRSRTS SFTEQLDEGTPNRESGDTQS FAQKLQLRVPSVESLFRSPI KESLFRSSSKESLVRTSSRE SLNRLDLDSSTASFDPPSDM DSEAEDLVGNSDSLNKEQLI QRLRRMERSL
Function Involved in vesicular trafficking at the Golgi apparatus level. May play a role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with MACF1. Involved in endosome-to-Golgi trafficking.
Protein Name Golgin Subfamily A Member 4
256 Kda Golgin
Golgin-245
Protein 72.1
Trans-Golgi P230
Database Links Reactome: R-HSA-6811440
Reactome: R-HSA-9673768
Cellular Localisation Cytoplasm
Golgi Apparatus Membrane
Peripheral Membrane Protein
Golgi Apparatus
Trans-Golgi Network Membrane
Alternative Antibody Names Anti-Golgin Subfamily A Member 4 antibody
Anti-256 Kda Golgin antibody
Anti-Golgin-245 antibody
Anti-Protein 72.1 antibody
Anti-Trans-Golgi P230 antibody
Anti-GOLGA4 antibody

Information sourced from Uniprot.org

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