| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Guanine nucleotide-binding protein subunit alpha-13 is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000ELISA 1:5000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GNA13 |
| Gene ID: |
10672 |
| Uniprot ID: |
GNA13_HUMAN |
| Specificity: |
GNA13 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: |
Synthesized peptide derived from part of the human protein |
| Post Translational Modifications | Palmitoylation is critical for proper membrane localization and signaling. Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation. |
| Function | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway. Inhibits CDH1-mediated cell adhesion in process independent from Rho activation. |
| Protein Name | Guanine Nucleotide-Binding Protein Subunit Alpha-13G Alpha-13G-Protein Subunit Alpha-13 |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-416482Reactome: R-HSA-428930Reactome: R-HSA-456926Reactome: R-HSA-9013148Reactome: R-HSA-9013149 |
| Cellular Localisation | Cell MembraneLipid-AnchorMelanosomeCytoplasmNucleusIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvDetected In The Cytoplasm Of Leydig Cells And In The Seminiferous EpitheliumIncluding Differentiating Cells From The Spermatogonia To Mature Spermatozoa StagesIn Round SpermatidsAlso Present In The Nuclei |
| Alternative Antibody Names | Anti-Guanine Nucleotide-Binding Protein Subunit Alpha-13 antibodyAnti-G Alpha-13 antibodyAnti-G-Protein Subunit Alpha-13 antibodyAnti-GNA13 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunohistochemistry analysis of ARHGEF1 in paraffin-embedded human tonsil using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/95232/377118/STJ116239_1__44864.1713144353.jpg?c=1 "Immunohistochemistry analysis of ARHGEF1 in paraffin-embedded human tonsil using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
