• Western blot analysis of extracts of various cell lines, using GMFB antibody (STJ111365) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of U-2 OS cells using GMFB Rabbit polyclonal antibody (STJ111365) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GMFB rabbit polyclonal antibody (STJ111365) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using GMFB Rabbit polyclonal antibody (STJ111365) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using GMFB rabbit polyclonal antibody (STJ111365) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GMFB Rabbit polyclonal antibody (STJ111365) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using GMFB rabbit polyclonal antibody (STJ111365) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using GMFB antibody (STJ111365) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GMFB antibody (1-140) (STJ111365)

SKU:
STJ111365

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GMFB (1-140) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GMFB
Gene ID: 2764
Uniprot ID: GMFB_HUMAN
Immunogen Region: 1-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human GMFB (NP_004115.1).
Immunogen Sequence: MSESLVVCDVAEDLVEKLRK FRFRKETNNAAIIMKIDKDK RLVVLDEELEGISPDELKDE LPERQPRFIVYSYKYQHDDG RVSYPLCFIFSSPVGCKPEQ QMMYAGSKNKLVQTAELTKV FEIRNTEDLTEEWLREKLGF
Post Translational Modifications Phosphorylated.stimulated by phorbol ester.
Function This protein causes differentiation of brain cells, stimulation of neural regeneration, and inhibition of proliferation of tumor cells.
Protein Name Glia Maturation Factor Beta
Gmf-Beta
Alternative Antibody Names Anti-Glia Maturation Factor Beta antibody
Anti-Gmf-Beta antibody
Anti-GMFB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance