Anti-Glutaminase antibody [R08-3D8] (STJA0034370)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein of human Glutaminase C
General Information
| Short Description | Rabbit monoclonal anti-Glutaminase for use in WB, ICC, IF and IP in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF/IP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R08-3D8 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | GLS |
| Gene ID | 2744 |
| Uniprot ID | GLSK_HUMAN |
| Immunogen | Recombinant protein of human Glutaminase C |
Additional Info
| Tissue Specificity | Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle. |
| Post Translational Modifications | Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits. |
| Function | Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain. Isoform 2: Lacks catalytic activity. |
| Protein Name | Glutaminase Kidney Isoform - MitochondrialGlsK-GlutaminaseL-Glutamine Amidohydrolase Cleaved Into - Glutaminase Kidney Isoform - Mitochondrial 68 Kda Chain - Glutaminase Kidney Isoform - Mitochondrial 65 Kda Chain |
| Database Links | Reactome: R-HSA-210500Reactome: R-HSA-5628897Reactome: R-HSA-8964539 |
| Cellular Localisation | Isoform 1: MitochondrionCytoplasmCytosolThe 74-Kda Cytosolic Precursor Is Translocated Into The Mitochondria And Processed Via A 72-Kda Intermediate To Yield The Mature 68- And 65-Kda SubunitsIsoform 3: MitochondrionGlutaminase Kidney IsoformMitochondrial 68 Kda Chain: Mitochondrion MatrixProduced By The Proteolytic Processing Of The 74-Kda Cytosolic PrecursorMitochondrial 65 Kda Chain: Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Glutaminase Kidney Isoform - Mitochondrial antibodyAnti-Gls antibodyAnti-K-Glutaminase antibodyAnti-L-Glutamine Amidohydrolase Cleaved Into - Glutaminase Kidney Isoform - Mitochondrial 68 Kda Chain - Glutaminase Kidney Isoform - Mitochondrial 65 Kda Chain antibodyAnti-GLS antibodyAnti-GLS1 antibodyAnti-KIAA0838 antibody |
Information sourced from Uniprot.org