• Anti-GLUD1 antibody (54-242) (STJ29946)
  • Immunoprecipitation analysis of 300 Mu g extracts of HepG2 cells using 3 Mu g GLUD1 antibody (STJ29946). Western blot was performed from the immunoprecipitate using GLUD1 antibody (STJ29946) at a dilution of 1:1000.
  • Immunofluorescence analysis of NIH-3T3 cells using GLUD1 Rabbit polyclonal antibody (STJ29946) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GLUD1 Rabbit polyclonal antibody (STJ29946) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of GLUD1 in paraffin-embedded rat kidney using GLUD1 Rabbit polyclonal antibody (STJ29946) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GLUD1 in paraffin-embedded mouse kidney using GLUD1 Rabbit polyclonal antibody (STJ29946) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GLUD1 in paraffin-embedded human liver using GLUD1 Rabbit polyclonal antibody (STJ29946) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GLUD1 in paraffin-embedded human colon carcinoma using GLUD1 Rabbit polyclonal antibody (STJ29946) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using GLUD1 Rabbit polyclonal antibody (STJ29946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GLUD1 antibody (54-242) (STJ29946)

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STJ29946

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GLUD1 (54-242) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GLUD1
Gene ID: 2746
Uniprot ID: DHE3_HUMAN
Immunogen Region: 54-242
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 54-242 of human GLUD1 (NP_005262.1).
Immunogen Sequence: SEAVADREDDPNFFKMVEGF FDRGASIVEDKLVEDLRTRE SEEQKRNRVRGILRIIKPCN HVLSLSFPIRRDDGSWEVIE GYRAQHSQHRTPCKGGIRYS TDVSVDEVKALASLMTYKCA VVDVPFGGAKAGVKINPKNY TDNELEKITRRFTMELAKKG FIGPGIDVPAPDMSTGEREM SWIADTYAS
Post Translational Modifications ADP-ribosylated by SIRT4, leading to inactivate glutamate dehydrogenase activity. Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer.
Function Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
Protein Name Glutamate Dehydrogenase 1 - Mitochondrial
Gdh 1
Database Links Reactome: R-HSA-2151201
Reactome: R-HSA-8964539
Cellular Localisation Mitochondrion
Endoplasmic Reticulum
Mostly Translocates Into The Mitochondria
Only A Small Amount Of The Protein Localizes To The Endoplasmic Reticulum
Alternative Antibody Names Anti-Glutamate Dehydrogenase 1 - Mitochondrial antibody
Anti-Gdh 1 antibody
Anti-GLUD1 antibody
Anti-GLUD antibody

Information sourced from Uniprot.org

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