| Host: |
Rabbit |
| Applications: |
WB/IHC-P/IF/ICC/IP/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GLUD1 |
| Gene ID: |
2746 |
| Uniprot ID: |
DHE3_HUMAN |
| Immunogen Region: |
54-242 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 54-242 of human GLUD1 (NP_005262.1). |
| Immunogen Sequence: |
SEAVADREDDPNFFKMVEGF FDRGASIVEDKLVEDLRTRE SEEQKRNRVRGILRIIKPCN HVLSLSFPIRRDDGSWEVIE GYRAQHSQHRTPCKGGIRYS TDVSVDEVKALASLMTYKCA VVDVPFGGAKAGVKINPKNY TDNELEKITRRFTMELAKKG FIGPGIDVPAPDMSTGEREM SWIADTYAS |
| Post Translational Modifications | ADP-ribosylated by SIRT4, leading to inactivate glutamate dehydrogenase activity. Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer. |
| Function | Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. |
| Protein Name | Glutamate Dehydrogenase 1 - MitochondrialGdh 1 |
| Database Links | Reactome: R-HSA-2151201Reactome: R-HSA-8964539Reactome: R-HSA-9837999 |
| Cellular Localisation | MitochondrionEndoplasmic ReticulumMostly Translocates Into The MitochondriaOnly A Small Amount Of The Protein Localizes To The Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Glutamate Dehydrogenase 1 - Mitochondrial antibodyAnti-Gdh 1 antibodyAnti-GLUD1 antibodyAnti-GLUD antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
