• Immunofluorescence analysis of A549 cells using GIF antibody (STJ28994). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using GIF antibody (STJ28994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GIF antibody (20-300) (STJ28994)

SKU:
STJ28994

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GIF (20-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CBLIF
Gene ID: 2694
Uniprot ID: IF_HUMAN
Immunogen Region: 20-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human GIF (NP_005133.2).
Immunogen Sequence: TQTQSSCSVPSAQEPLVNGI QVLMENSVTSSAYPNPSILI AMNLAGAYNLKAQKLLTYQL MSSDNNDLTIGQLGLTIMAL TSSCRDPGDKVSILQRQMEN WAPSSPNAEASAFYGPSLAI LALCQKNSEATLPIAVRFAK TLLANSSPFNVDTGAMATLA LTCMYNKIPVGSEEGYRSLF GQVLKDIVEKISMKIKDNGI IGDIYSTGLAMQALSVTPEP SKKEWNCKKTTDMILNEIK
Tissue Specificity Gastric mucosa.
Function Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the CBLIF-cobalamin complex is internalized via receptor-mediated endocytosis.
Protein Name Cobalamin Binding Intrinsic Factor
Gastric Intrinsic Factor
Intrinsic Factor
If
Inf
Database Links Reactome: R-HSA-3359457
Reactome: R-HSA-3359462
Reactome: R-HSA-3359463
Reactome: R-HSA-9758881
Cellular Localisation Secreted
Alternative Antibody Names Anti-Cobalamin Binding Intrinsic Factor antibody
Anti-Gastric Intrinsic Factor antibody
Anti-Intrinsic Factor antibody
Anti-If antibody
Anti-Inf antibody
Anti-CBLIF antibody
Anti-GIF antibody
Anti-IFMH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance