| Host: |
Rabbit |
| Applications: |
WB/IHC-P/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GEMIN5 |
| Gene ID: |
25929 |
| Uniprot ID: |
GEMI5_HUMAN |
| Immunogen Region: |
750-950 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 750-950 of human GEMIN5 (NP_056280.2). |
| Immunogen Sequence: |
RTPVKLESIDGNEEESMKEN SGPVENGVSDQEGEEQAREP ELPCGLAPAVSREPVICTPV SSGFEKSKVTINNKVILLKK EPPKEKPETLIKKRKARSLL PLSTSLDHRSKEELHQDCLV LATAKHSRELNEDVSADVEE RFHLGLFTDRATLYRMIDIE GKGHLENGHPELFHQLMLWK GDLKGVLQTAAERGELTDNL V |
| Function | The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Within the SMN complex, GEMIN5 recognizes and delivers the small nuclear RNAs (snRNAs) to the SMN complex. Binds to the 7-methylguanosine cap of RNA molecules. Binds to the 3'-UTR of SMN1 mRNA and regulates its translation.does not affect mRNA stability. May play a role in the regulation of protein synthesis via its interaction with ribosomes. |
| Protein Name | Gem-Associated Protein 5Gemin5 |
| Database Links | Reactome: R-HSA-191859Reactome: R-HSA-9754678 |
| Cellular Localisation | NucleusNucleoplasmGemCytoplasmFound Both In The Nucleoplasm And In Nuclear Bodies Called Gems (Gemini Of Cajal Bodies) That Are Often In Proximity To Cajal (Coiled) BodiesAlso Found In The Cytoplasm |
| Alternative Antibody Names | Anti-Gem-Associated Protein 5 antibodyAnti-Gemin5 antibodyAnti-GEMIN5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
